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FSSAI asks labs to use newly-approved methods for testing fortificants.
Sep 19, 2022

The Food Authority has asked food laboratories to use newly-approved methods for testing fortificants including iron, folic acid, and vitamin B12 in fortified rice.
 
The FSSAI has issued an order in this regard, stating, ‘the food testing laboratories are hereby requested to use the methods with immediate effect’.
 
For iron in fortified rice, the Method was applicable for Quantification of Iron at 10 ppm LOQ Level (with respect to the Sample) by using ICP-MS method with Limit of Detection at 4 mg/kg and Limit of Quantification 10 mg/kg respective to the Sample.
 
For determination of Folic Acid (Vitamin B9) in Fortified Rice, the Method was applicable for Quantification of Folic Acid (Vitamin B9) at 10 ppb LOQ Level (with respect to the Sample) by using LC-MS/MS method in Fortified Rice wherein Limit of Detection is 5 ppb  and Limit of Quantification is 10 ppb respective to the Sample.
 
For determination of Cyanocobalamin (Vitamin B12) in Fortified Rice, the method includes for Quantification of Cyanocobalamin (Vitamin B12) at 0.5 ppb LOQ Level (with respect to the Sample) by using LC-MS/MS method with a Limit of Detection at 0.25 µg/kg and Limit of Quantification at 0.5 µg/kg with respect to the Sample.
 
The FSSAI order also details the preparation of test samples while the labs were asked to use freshly prepared standard solutions for the analysis.
    
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