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FSSAI issues manual of methods of analysis of honey, seeks comments.
May 26, 2023

The FSSAI has issued a manual of methods of analysis of honey and sought comments from stakeholders on the same.  
The methods include determination of specific gravity, moisture, total reducing sugars, sucrose, fructose, total ash, acidity, hydroxyl methyl furfural, water insoluble matters and pollen and plant elements.  
According to the FSSAI, the test methods given in the manual are standardised / validated and were taken from national or international methods or recognised specifications, however it would be the responsibility of the respective testing laboratory to verify the performance of these methods onsite and ensure that it gives proper results before putting these methods in to use.
Meanwhile, the manual defines specific gravity as the ratio of the density of a substance to that of a standard substance. The determination of specific gravity shall be for all types of honey including carvia callosa and honey dew.  
The determination of Total Reducing Sugars, Sucrose and Fructose-Glucose Ratio (Titrimetric Method) looks for the difference in the concentrations of invert sugar multiplied by 0.95 to give the apparent sucrose content.
The free acidity is defined as the acidity titratable with sodium hydroxide up to the equivalence point.
And amongst other, diastase is defined as an enzyme that is found naturally in honey and degrades over time, especially when exposed to heat.